<p dir="ltr">This study used a novel approach combining stable isotope data and condensed multivariate fatty acid data to define comparative niche space and overlap of six sympatric sharks from the southwest Indian Ocean: <i>Galeocerdo cuvier</i>, <i>Sphyrna zygaena</i>, <i>S. lewini</i>, <i>Carcharias taurus</i>, <i>Carcharodon carcharias</i>, and <i>Carcharhinus obscurus</i>.</p>