Toehold-Mediated Nonenzymatic DNA Strand Displacement analysis dataset

This dataset comprises the results of analysis of toehold-mediated DNA strand displacement. The results demonstrate a new, simpler approach for the analysis of real-life DNA samples using a toehold-mediated DNA strand displacement reaction where a partially complementary duplex, with a ss-toehold sequence, was used as a target for analysis.<br><br>The approach was successfully implemented for human DNA genotyping for gender identification and it is envisaged that this model system could be easily adapted to other DNA genotyping molecular diagnostics.<br><br>Human genomic and mitochondrial DNA were coextracted from the author’s own blood samples. The analysis of this DNA consisted of two stages:<br>(i) PCR amplification was used to generate a PCR product with a contiguous ss-toehold domain; and<br>(ii) toehold-mediated DNA strand displacement reactions between the target toehold-PCR product and single-stranded chemically synthesized oligonucleotide molecular probes were performed using a quenched Föster resonance energy transfer (FRET) technique to monitor how the reaction proceeded.<div>Copyright (c) 2013 Flinders University, South Australia<br><br>Date coverage: 2012-01-01 - 2012-06-01</div>