File(s) not publicly available
Toehold-Mediated Nonenzymatic DNA Strand Displacement analysis dataset
dataset
posted on 2021-11-01, 05:05 authored by Dmitriy Khodakov, Anastasia Khodakova, Adrian LinacreAdrian Linacre, Amanda EllisThis dataset comprises the results of analysis of toehold-mediated DNA strand displacement. The results demonstrate a new, simpler approach for the analysis of real-life DNA samples using a toehold-mediated DNA strand displacement reaction where a partially complementary duplex, with a ss-toehold sequence, was used as a target for analysis.
The approach was successfully implemented for human DNA genotyping for gender identification and it is envisaged that this model system could be easily adapted to other DNA genotyping molecular diagnostics.
Human genomic and mitochondrial DNA were coextracted from the author’s own blood samples. The analysis of this DNA consisted of two stages:
(i) PCR amplification was used to generate a PCR product with a contiguous ss-toehold domain; and
(ii) toehold-mediated DNA strand displacement reactions between the target toehold-PCR product and single-stranded chemically synthesized oligonucleotide molecular probes were performed using a quenched Föster resonance energy transfer (FRET) technique to monitor how the reaction proceeded.
The approach was successfully implemented for human DNA genotyping for gender identification and it is envisaged that this model system could be easily adapted to other DNA genotyping molecular diagnostics.
Human genomic and mitochondrial DNA were coextracted from the author’s own blood samples. The analysis of this DNA consisted of two stages:
(i) PCR amplification was used to generate a PCR product with a contiguous ss-toehold domain; and
(ii) toehold-mediated DNA strand displacement reactions between the target toehold-PCR product and single-stranded chemically synthesized oligonucleotide molecular probes were performed using a quenched Föster resonance energy transfer (FRET) technique to monitor how the reaction proceeded.
Copyright (c) 2013 Flinders University, South Australia
Date coverage: 2012-01-01 - 2012-06-01
Date coverage: 2012-01-01 - 2012-06-01
